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IICBU Biological Image Repository

The purpose of this image repository is to provide a benchmark for testing and comparing the performance of image analysis algorithms for biological imaging.

For more information, please refer to: Shamir, L., Orlov, N., Eckley, D.M., Macura, T., Goldberg, I.G.; IICBU 2008 - A Proposed Benchmark Suite for Biological Image Analysis, Medical & Biological Engineering & Computing, vol. 46, no. 9, pp. 943-947, 2008 [local PDF]

Image Datasets --

DatasetShort Description # of classes # of images Image format MicroscopyContributor
PollenGeometric features of pollen grains 7 630 25x25 8 bit TIFF Phase contrast Duller, A.W.G., Duller, G.A.T., France, I. and Lamb, H.F download
(0.36MB)
RNAiRNAi library of 16 phenotypes of drosophila cells with single gene knockdowns 10 200 1024x1024 16 bit TIFF Fluorescence Mark Eckley download
(220MB)
C. elegans muscle ageImages of C. elegans muscles at different ages 4 252 1600x1200 16 bit TIFF Fluorescence Nikita Orlov, Wendy Iser, Cathy Wolkow download
(612MB)
Terminal bulb agingImages of C. elegans terminal bulb at different ages 7 970 300x300 16 bit TIFF DIC Josiah Johnston, Cathy Wolkow download
(67MB)
BinucleateDAPI images of binucleate and regular cells 2 40 1280x1024 16 bit TIFF Fluorescence Aaron Straight download
(59MB)
LymphomaMalignant lymphoma 3 375 1388x1040 32 bit TIFF (color) Brightfield Elaine Jaffe, Nikita Orlov download
(381MB)
Liver gender (CR)Liver tissue sections from male and female mice on caloric restriction diet 2 256 1388x1040 32 bit TIFF (color) Brightfield Tomasz Macura, Suresh Poosala download
(2.2GB)
Liver gender (AL)Liver tissue sections from male and female mice on ad-libitum diet 2 522 1388x1040 32 bit TIFF (color) Brightfield Tomasz Macura, Suresh Poosala download
(2.1GB)
Liver AgingLiver tissue sections from an aging study involving female mice on ad-libitum or caloric restriction diets 4 850 1388x1040 32 bit TIFF (color) Brightfield Tomasz Macura, Suresh Poosala download
(4.5GB)
2D HeLaFluorescence microscopy images of HeLa cells using 10 different labels 10 860 382x382 16 bit TIFF Fluorescence Robert F. Murphy download
(39MB)
CHOFluorescence microscopy images of different sub-cellular compartments 5 340 512x382 16 bit TIFF Fluorescence Robert F. Murphy download
(11MB)
WND-CHARM Classification Accuracy



* WND-CHARM is released under the GPL. Its source-code is available here
More about WND_CHARM can be found at: Shamir, L., Orlov, N., Eckley, D. M., Macura, T., Johnston, J., Goldberg, I.; Wndchrm - An Open Source Utility for Biological Image Analysis. BMC - Source Code for Biology and Medicine, vol. 3, 13, 2008.



If you have technical questions or willing to contribute a dataset, please contact Lior Shamir shamirl at mail dot nih dot gov

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For More Information
Ilya Goldberg, Ph.D. igg at nih dot gov
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Updated: Tuesday September 12, 2007